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Although you may need homogeneous, pure template for initial copy number determination, for generation of the standard curve, it is best to use target template that is as similar to the experimental samples as possible.

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Protocol uses javascript. Monitor reactions in real time. How PCR works step by step? Start PCR Touch-down PCR adding detergents reducing the PCR cycles or. Taq polymerase to build the complementary strand.

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PCR and multiplex approaches. DNA by melting curve analysis. Thank you for your answers. Obviously, it can save time. This variation may increase with age and usage of the instrument. Document for future monitoring activities.

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These results also demonstrate that when both the cycling conditions are correctly designed and the reagents are at optimal concentrations, the PCR experiment produces a discreet amplicon corresponding to the expected size.

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This is the initial assessment of primer and probe design.